Studies on glucosaminidase. 2. Substrates for N-acetyl-beta-glucosaminidase.

Is urinary N-acetyl-beta-D-glucosaminidase a marker of urological abnormality in children?

  Abstract   Background: Hydronephrosis is the most common congenital condition that is detected   by prenatal ultrasonography. Moreover, the widespread use of prenatal ultrasonography   results in an increased recognition of fetal hydronephrosis. Prenatal hydronephrosis   is diagnosed at an incidence of 1:100 to 1:500 by ultrasonographic studies. The presence of hydronephrosis is not synonymou...

Purification, biochemical properties and active sites of N-acetyl-beta-D-hexosaminidases from human seminal plasma.

Two isoenzymes of N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30) (Hex A and Hex B) from human seminal plasma were purified to homogeneity with specific activities of 26 and 60 units/mg of protein respectively. N-Acetyl-beta-D-glucosaminidase activity was inseparable from N-acetyl-beta-D-galactosaminidase activity in both Hex A and Hex B by various conventional chromatographic procedures. Althoug...

Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A & B in workers exposed to cadmium at cadmium plating

OBJECTIVE The present study was carried out to determine the effect of cadmium exposure on Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B in workers exposed at cadmium plating. METHODS 50 subjects using cadmium during cadmium plating formed the study group. An equal number of age-sex matched subjects working in administrative section formed the control group. Urinary cadm...

Studies on glucosaminidase; N-acetyl-beta-glucosaminidase in rat kidney.

a-Glucosidase and N-Acetyl-p-o-glucosaminidase Isoenzymes in Serum

Usingdifferent conditions for incubation and fluorometry with 4-methylumbelliferylglycosides as substrates, we demonstratedthe presence ofacida-glucosidase, “renal” a-glucosidase, N-acetyl--D-glucosaminidase A, and N-acetyl-f3-o-glucosaminidase B in freshly drawn normal human serum. The acid a-glucosidase enzymatic activity was determined at pH 4.0 in 0.1 mol/L Tris reagent, whereasthe renal is...

Excretion of urinary N-acetyl-beta-D-glucosaminidase isoenzymes after renal transplantation in the rat.

The urinary excretion of N-acetyl-beta-D-glucosaminidase isoenzymes A and B following kidney transplantation was studied in rats. High enzymuria with permanent marked isoenzyme B excretion occurred from the immediate post-operative period to the irreversible rejection episode. Isoenzyme B could represent as much as 10-40% of total N-acetyl-beta-D-glucosaminidase activity and it reflected the in...